Simulated Atmospheric Nitrogen Deposition Alters Actinobacterial Community Composition in Forest Soils

TitleSimulated Atmospheric Nitrogen Deposition Alters Actinobacterial Community Composition in Forest Soils
Publication TypeJournal Article
Year of Publication2010
AuthorsEisenlord SD, Zak DR
JournalSoil Science Society of America Journal
Volume74
Issue4
Pagination1157
Date Published2010
Abstract

Anthropogenic N deposition can slow the decay of plant detritus, leading to an accumulation of soil organic matter and the production of phenolic dissolved organic C (DOC), which can leach from soil to ground and surface waters. Actinobacteria are one of the few groups of saprotrophic microorganisms that oxidatively depolymerize lignin, producing substantial soluble polyphenolics in the process. In combination, these observations present the possibility that lignolytic Actinobacteria may become more important agents of lignin decay as atmospheric N deposition continues to increase during the next decade. To test this idea, we quantified actinobacterial abundance and community composition in a well-replicated fi eld study in which atmospheric N deposition has been experimentally increased since 1994. Actinobacterial abundance was assessed using quantitative polymerase chain reaction of 16S rRNA and community composition was evaluated using clone libraries and phylogenetic community analyses (i.e., LIBSHUFF and UniFrac). Contrary to our expectation, experimental atmospheric N deposition had no eff ect on actinobacterial abundance in the forest fl oor (~1010 gene copies kg−1); however, it significantly decreased actinobacterial abundance by 47% and total DNA by 31% in surface soil. Our analyses revealed that experimental N deposition further elicited a significant membership change in forest floor and surface soil communities, as well as significant differences in the phylogenetic diversity of forest floor Actinobacteria. Th is shift in community composition occurred in concert with a slowing of plant litter decay, accumulation of soil organic matter, and a greater production of phenolic DOC. These observations are consistent with the idea that changes in actinobacterial community composition may underlie biogeochemical responses to experimental N deposition.

DOI10.2136/sssaj2009.0240